A cell-based screening system for anti-influenza A virus agents
Identifieur interne : 000601 ( Main/Exploration ); précédent : 000600; suivant : 000602A cell-based screening system for anti-influenza A virus agents
Auteurs : Wan Ying Wong [Malaisie] ; Sheng Wei Loh [Malaisie] ; Wei Lun Ng [Malaisie] ; Ming Cheang Tan [Malaisie] ; Kok Siong Yeo [Malaisie] ; Chung Yeng Looi [Malaisie] ; Mohd Jamil Maah [Malaisie] ; Chee-Kwee Ea [Malaisie]Source :
- Scientific Reports [ 2045-2322 ] ; 2015.
Abstract
Emerging of drug resistant influenza A virus (IAV) has been a big challenge for anti-IAV therapy. In this study, we describe a relatively easy and safe cell-based screening system for anti-IAV replication inhibitors using a non-replicative strain of IAV. A nickel (II) complex of polyhydroxybenzaldehyde N4-thiosemicarbazone (NiPT5) was recently found to exhibit anti-inflammatory activity
Url:
DOI: 10.1038/srep08672
PubMed: 25728279
PubMed Central: 4345322
Affiliations:
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cell-based screening system for anti-influenza A virus agents</title><author><name sortKey="Wong, Wan Ying" sort="Wong, Wan Ying" uniqKey="Wong W" first="Wan Ying" last="Wong">Wan Ying Wong</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Institute of Biological Sciences, Faculty of Science, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Loh, Sheng Wei" sort="Loh, Sheng Wei" uniqKey="Loh S" first="Sheng Wei" last="Loh">Sheng Wei Loh</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Institute of Biological Sciences, Faculty of Science, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ng, Wei Lun" sort="Ng, Wei Lun" uniqKey="Ng W" first="Wei Lun" last="Ng">Wei Lun Ng</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Institute of Biological Sciences, Faculty of Science, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Tan, Ming Cheang" sort="Tan, Ming Cheang" uniqKey="Tan M" first="Ming Cheang" last="Tan">Ming Cheang Tan</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Institute of Biological Sciences, Faculty of Science, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yeo, Kok Siong" sort="Yeo, Kok Siong" uniqKey="Yeo K" first="Kok Siong" last="Yeo">Kok Siong Yeo</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Institute of Biological Sciences, Faculty of Science, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Looi, Chung Yeng" sort="Looi, Chung Yeng" uniqKey="Looi C" first="Chung Yeng" last="Looi">Chung Yeng Looi</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Pharmacology, Faculty of Medicine, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Maah, Mohd Jamil" sort="Maah, Mohd Jamil" uniqKey="Maah M" first="Mohd Jamil" last="Maah">Mohd Jamil Maah</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Department of Chemistry, Faculty of Science, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ea, Chee Kwee" sort="Ea, Chee Kwee" uniqKey="Ea C" first="Chee-Kwee" last="Ea">Chee-Kwee Ea</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Institute of Biological Sciences, Faculty of Science, University of Malaya</institution>, 50603 Kuala Lumpur,<country>Malaysia</country></nlm:aff><country xml:lang="fr">Malaisie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Emerging of drug resistant influenza A virus (IAV) has been a big challenge for anti-IAV therapy. In this study, we describe a relatively easy and safe cell-based screening system for anti-IAV replication inhibitors using a non-replicative strain of IAV. A nickel (II) complex of polyhydroxybenzaldehyde N4-thiosemicarbazone (NiPT5) was recently found to exhibit anti-inflammatory activity <italic>in vivo</italic> and <italic>in vitro</italic>. NiPT5 impedes the signaling cascades that lead to the activation of NF-κB in response to different stimuli, such as LPS and TNFα. Using our cell-based screening system, we report that pretreating cells with NiPT5 protects cells from influenza A virus (IAV) and vesicular stomatitis virus (VSV) infection. Furthermore, NiPT5 inhibits replication of IAV by inhibiting transcription and translation of vRNAs of IAV. Additionally, NiPT5 reduces IAV-induced type I interferon response and cytokines production. Moreover, NiPT5 prevents activation of NF-κB, and IRF3 in response to IAV infection. These results demonstrate that NiPT5 is a potent antiviral agent that inhibits the early phase of IAV replication.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Samji, T" uniqKey="Samji T">T. Samji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szatraj, K" uniqKey="Szatraj K">K. Szatraj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ison, M G" uniqKey="Ison M">M. G. Ison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bavin, E M" uniqKey="Bavin E">E. M. Bavin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamre, D" uniqKey="Hamre D">D. Hamre</name></author><author><name sortKey="Brownlee, K A" uniqKey="Brownlee K">K. A. Brownlee</name></author><author><name sortKey="Donovick, R" uniqKey="Donovick R">R. 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Ting</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Malaisie</li></country></list><tree><country name="Malaisie"><noRegion><name sortKey="Wong, Wan Ying" sort="Wong, Wan Ying" uniqKey="Wong W" first="Wan Ying" last="Wong">Wan Ying Wong</name></noRegion><name sortKey="Ea, Chee Kwee" sort="Ea, Chee Kwee" uniqKey="Ea C" first="Chee-Kwee" last="Ea">Chee-Kwee Ea</name><name sortKey="Loh, Sheng Wei" sort="Loh, Sheng Wei" uniqKey="Loh S" first="Sheng Wei" last="Loh">Sheng Wei Loh</name><name sortKey="Looi, Chung Yeng" sort="Looi, Chung Yeng" uniqKey="Looi C" first="Chung Yeng" last="Looi">Chung Yeng Looi</name><name sortKey="Maah, Mohd Jamil" sort="Maah, Mohd Jamil" uniqKey="Maah M" first="Mohd Jamil" last="Maah">Mohd Jamil Maah</name><name sortKey="Ng, Wei Lun" sort="Ng, Wei Lun" uniqKey="Ng W" first="Wei Lun" last="Ng">Wei Lun Ng</name><name sortKey="Tan, Ming Cheang" sort="Tan, Ming Cheang" uniqKey="Tan M" first="Ming Cheang" last="Tan">Ming Cheang Tan</name><name sortKey="Yeo, Kok Siong" sort="Yeo, Kok Siong" uniqKey="Yeo K" first="Kok Siong" last="Yeo">Kok Siong Yeo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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